Year: 2018 | Month: June | Volume 8 | Issue 3
Virulence and Antibiogram Study of Escherichia coli Isolated from Faecal Sample of Buffalo
Abstract:
The study was undertaken for detection of virulence gene using polymerase chain reaction (PCR) and investigate their antibiotic sensitivity pattern in fecal sample of buffalo. In present study, a total of 180 samples were processed for isolation of verocytotoxic E. coli (VTEC). Out of 180 samples, 19 samples were found positive for VTEC. All VTEC strains were subjected to antibiotic sensitivity test using 16 antibiotic discs. Of these, ciprofoxacin and imipenem were found highly sensitive (100%) against VTEC strains followed by ceftriaxone (89.47%), gentamicin (84.21%), amikacin (84.21%) and chloramphenicol (78.94%). Least sensitivity was recorded for enrofloxacin (78.94%), followed by cefixime (63.15%), erythromycin (57.84%) and tetracycline (52.63%).
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